I want to draw a picture of a plasmid, something along the lines of this image. i have tried all of the free software i could find does anyone have any suggestions of software that would produce an image this good, preferably free but I’ll pay if I have to. If you’re willing to use HTML syntax, you can design something pretty close using http://angularplasmid.vixis.com. It’s free, open-source, and comes with plenty of examples of how to create what you’re looking for. See below for examples: The best-known software for this kind of task is commercial, namely DNASTAR’s Lasergene Seqbuilder and Invitrogen’s Vector NTI. Another open-source software alike is UGENE. It has plasmid annotation functions, restriction analysis, cloning framework, etc. This is probably not what you are looking for, but if you want a hands on coding solution, Biopython’s Genome Diagram can do something a bit like that. There is a basic example in the Tutorial, http://www.biopython.org/DIST/docs/tutorial/Tutorial.html#htoc196 You got the free Web interface MGV2 which I find really easy to use and gives nice pics. Login as ‘guest’ to give it a try I’m surprised Circos hasn’t been mentioned so far. The microbial genome recipe could be a good starting point.. Gene Construction Kit® (GCK) is a useful tool for plasmid mapping, cloning simulation, and DNA analysis. It has been consistently reviewed as being one of the easiest to use applications for simulating multi-step cloning operations, and offers extensive flexibility in the graphical formatting of DNA sequences – and – generating those colorful, customized plasmid maps seen in publications. You can easily copy and paste graphical maps from GCK to image editing programs, or even create hi-res PDF (Mac) or XPS (Win) exports from within the program. It’s been around since the mid-90’s, and was one of, if not ‘the very first software’ for performing these types of graphical manipulations of DNA sequence data. Try WebDSV, it is a free web app, cross-platform, no registration needed, GenBank file format compatible. Source.