If this is the first time that you have started vectorNTI you will be asked a bunch of questions about what to install. I suggest that you install everything to begin with, because you can always remove stuff later. The databases store all the sequence data. There are different databases for DNA sequences, protein sequences, oligos and so on. Click on the pull down menu to see the databases available Each database can also contain sub-databases. You can have many different subbases, for example, one for each user. You can keep a shorter list of sequences in your own subbase. All the sequences will appear in the main database. You can enter sequences into your own database, either by dragging them from the main window, or by opening new sequences. Suppose that you want to download a sequence from NCBI and enter it into your own subbase. These are the steps that you will follow: Note: You can also download sequences directly from NCBI into vectorNTI either by choosing to open a sequence using VNTIMolDoc from the download manager or within vectorNTI. I am not going to describe these techniques here because this is the most important technique. It will allow you to enter sequences from a variety of sources. Source.