Construction of a novel shuttle vector for use in Haemophilus influenzae and H. parainfluenzae

(*) conjugation efficiency is expressed in different units from transformation efficiency, as conjugation does not involve introduction of a measurable quantity of DNA into a reaction. The only measurable input is donor cells. Frequency of fimbriation of nontypable Haemophilus influenzae and its ability to adhere to chinchilla and human respiratory epithelium Site-specific recombination with the chromosomal tRNA(Leu) gene by the large conjugative Haemophilus resistance plasmid An analysis of the complete nucleotide sequence of the Haemophilus ducreyi broad-host-range plasmid pLS88 Characterization and modeling of the Haemophilus influenzae core and supragenomes based on the complete genomic sequences of Rd and 12 clinical nontypeable strains A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR J.A. Nickoloff (Ed.), Electroporation Protocols for Microorganisms, Humana press, Totowa, N.J. (1995) Electroporation of Haemophilus influenzae is effective for transformation of plasmid but not chromosomal DNA Transferable antibiotic resistance elements in Haemophilus influenzae share a common evolutionary origin with a diverse family of syntenic genomic islands A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria Specificity of human bactericidal antibodies against PorA P1.7, 16 induced with a hexavalent meningococcal outer membrane vesicle vaccine Source.

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