Efficient Designer Nuclease-Based Homologous Recombination Enables Direct PCR Screening for Footprintless Targeted Human Pluripotent Stem Cells

A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability Cytokine production using membrane adsorbers: Human basic fibroblast growth factor produced by Escherichia coli Sequential genetic modification of the hprt locus in human ESCs combining gene targeting and recombinase-mediated cassette exchange A targeted NKX2.1 human embryonic stem cell reporter line enables identification of human basal forebrain derivatives Shuttle system allowing simplified cloning of expression cassettes into advanced generation lentiviral vectors Expanding or restricting the target site repertoire of zinc-finger nucleases: the inter-domain linker as a major determinant of target site selectivity Directing cardiomyogenic differentiation of human pluripotent stem cells by plasmid-based transient overexpression of cardiac transcription factors Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases Highly efficient zinc-finger nuclease-mediated disruption of an eGFP transgene in keratinocyte stem cells without impairment of stem cell properties Differential integrity of TALE nuclease genes following adenoviral and lentiviral vector gene transfer into human cells Insertional transformation of hematopoietic cells by self-inactivating lentiviral and gammaretroviral vectors A novel TALE nuclease scaffold enables high genome editing activity in combination with low toxicity Selection-independent generation of gene knockout mouse embryonic stem cells using zinc-finger nucleases In situ genetic correction of the sickle cell anemia mutation in human induced pluripotent stem cells using engineered zinc finger nucleases Robust, persistent transgene expression in human embryonic stem cells is achieved with AAVS1-targeted integration Generation of isogenic pluripotent stem cells differing exclusively at two early onset Parkinson point mutations Genomic instability and myelodysplasia with monosomy 7 consequent to EVI1 activation after gene therapy for chronic granulomatous disease Identification of genes involved in growth autonomy of hematopoietic cells by analysis of factor-independent mutants Structure-based redesign of the dimerization interface reduces the toxicity of zinc-finger nucleases Oxidase-deficient neutrophils from X-linked chronic granulomatous disease iPS cells: functional correction by zinc finger nuclease-mediated safe harbor targeting This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. Source.

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