ABOUT SHEILA With my degree in Biology, I spent the last 9 years as a Graphic Designer at research laboratories in the US and Switzerland, working extensively with post-docs and graduate students. I have a lot of experience helping them optimize their images for publication, and I would like to share it with you. I like to use Adobe Illustrator (AI) for page layout. It can handle multiple text boxes, multiple images, and you can draw schematics right on the page. It can also be used for multiple page layouts. If you decide to use AI as your page layout program, you will need to understand the difference between an embedded image and a linked image. Here are some images to further explain. In AI, I opened the File drop-down menu and clicked on Place. The Place dialog box appeared: From this box I chose the image that I was interested in placing on the AI page. I highlighted the check-box that you need to pay attention to when creating your own files. If the Link option is checked, you’ll link the file. If you don’t check the Link option box then the file will be embedded. In this image I placed the same file two times. For the first image I didn’t check the Link option and for the second image I did check the Link box, therefore, the first image is embedded and the second image is linked. From the Window drop-down you can click Links and open the links window. This will show all of your placed items, links AND embedded images. In this screenshot, I highlighted the icon that shows you the image file is embedded. The bottom Image2.jpg is the linked file and doesn’t have an icon next to it. After placing the images above, I closed the file. Then I moved the image file (Image2.jpg) to a different location. When I re-opened Adobe Illustrator, it didn’t know where to find the linked file. It gave me an error message: At this point, if you know where the linked image file got moved to, you could click replace and let the program know where to find it. Otherwise, you can choose Ignore , but then the file won’t be a part of your page. As you can see in the next image, I chose Ignore. The embedded image shows up perfectly, but the second copy (the linked one) is missing. If you open the links menu again you will see the red question mark icon, indicating the broken link. For the final example in this series, I opened the Image2.jpg file and changed the colors from green to red. Then I re-opened the Adobe Illustrator file. When I was confronted with the dialog box telling me that it couldn’t find the linked file, I chose Replace. I clicked through locations and picked the new location for the Image2.jpg file. This is the result: Source.