Print: Print the vector map, the sequence, the detailed analysis result, and the sequence notes added. . The donor vector and the expression vector are required for the BP reaction and for generating an entry clone. An entry vector and a destination vector are required for the LR reaction and for generating an expression vector. experiments. The TA cloning wizard accepts both commercial T-vectors or restriction designed T-vectors and combines them with the modified PCR product. You can then generate a highly accurate recombinant DNA plasmid map. : The cloning technique requires restriction enzymes to cut the vector molecule and the molecule to be cloned. SimVector performs restriction enzyme analysis and allows you to filter, annotate and map the restriction enzymes on the desired sequences. : A comprehensive database of over 1000 restriction enzymes is used to perform restriction analysis. SimVector identifies restriction enzyme recognition sites in DNA sequences for mapping restriction enzymes. Two modes of enzyme filtering are provided to specify the parameters for restriction analysis. Enzyme filtering enables you to set the enzyme criteria to match your experimental requirements. For convenience, enzyme sets from more than 20 commercial providers are included. : SimVector identifies the MCS's by interpreting GenBank header annotations when available, or you can specify them manually. It even has the smarts to list only the enzymes that cut within the MCS and nowhere else. This special format we call the Bracket View. SimVector generates plasmid maps in vector graphic format for exporting to Adobe Illustrator 10 and Microsoft PowerPoint 2002. Exports high resolution plasmid map images in JPEG, PNG and TIF format for easy addition to web sites and exports images are ready to host pages. Choose from hundreds of fonts, colors, styles and fill patterns for restriction enzymes and features with a click of a button. : You can find ORFs in the chosen reading frames, identified with the selected start and stop codons, and compliant to the specified minimum length. With a click of a button, you can now ensure that the construct remains in-frame after cloning. The graphical ORF display is always ready for reference and further analysis. on complete sequences as well as on fragments by specifying a range. The results of the analysis are mapped on the plasmid maps, displaying the positions of restriction enzyme cleavage sites. : Restriction mapping is the first step in planning a cloning experiment. SimVector offers complete control over all key parameters to perform the restriction enzyme analysis. SimVector makes restriction mapping easy. Just select the enzymes you would like to use and let SimVector draw a high quality restriction site map. SimVector analyzes your maps for over a 1000 Source.