To expedite publication of your paper, please follow these style guidelines in preparing your figures for your revised manuscript. Note that some of these instructions (with respect to format and resolution) differ from the instructions for figures with initial manuscript submission. [Important note: We cannot accept figures in prepared in Microsoft PowerPoint at the revision stage! Please adhere to the formatting guidelines in this document.] Resolution. For manuscripts in the revision stage, adequate figure resolution is essential to a high-quality print and online rendering of your paper. Raster line art should have a minimum resolution of 600 dots per inch (dpi) and, preferably, should have a resolution of 1200 dpi. Grayscale and color artwork should have a minimum resolution of 400 dpi, and a higher resolution if possible. These resolutions apply to figures sized at dimensions comparable to those of figures in the print journal. Reducing or enlarging the dimensions of a digital raster image will also change its resolution. For example, reducing the dimensions of an image by 50%, with no change in file size, will double its dpi resolution, doubling the dimensions of the image will cut resolution by 50%. Authors are encouraged to review past issues to gauge the approximate size their figures will take in the print publication, and set the resolution of their figures accordingly. Format. Electronic figure files at the revision stage must be in one of the following formats: Adobe Portable Document Format (PDF), Encapsulated PostScript (EPS), or Adobe Illustrator (AI) for vector illustrations or diagrams, Tagged Image File Format (TIFF) (minimum 300 dpi) for raster illustrations and diagrams, EPS or PDF for vector and raster combinations, Tagged Image File Format (TIFF), (minimum 300 dpi) for raster photograph or microscopy images. Authors who have created their files using Adobe Illustrator or Adobe Photoshop should provide their files in these native file formats. Modification of figures. Science does not allow certain electronic enhancements or manipulations of micrographs, gels, or other digital images. Figures assembled from multiple photographs or images, or non-concurrent portions of the same image, must indicate the separate parts with lines between them. Linear adjustment of contrast, brightness, or color must be applied to an entire image or plate equally. Nonlinear adjustments must be specified in the figure legend. Selective enhancement or alteration of one part of an image in not acceptable. In addition, Science may ask authors of papers returned for revision to provide additional documentation of their primary data. [Note: Most of these suggestions also appear in our tips for preparing efficient figures for initial submission.] Use solid symbols for plotting data if possible (unless data overlap or there are multiple symbols). Size symbols so that they will be distinguishable when the figure is reduced. Line widths should be legible upon reduction (minimum of 0.5 pt at the final reduced size). If you are reproducing images from another source or do not own the copyright on those images, we must have permission to publish in print and online. Copyright 2016 Science/AAAS. American Association for the Advancement of Science. All Rights Reserved.AAAS is a partner of HINARI, AGORA, OARE, PatientInform, CrossRef, and COUNTER. Source.