Transformation of Stomoxys calcitrans with a Hermes gene vector | DeepDyve

Enjoy unlimited access and personalized recommendations from over 12 million articles from more than 10,000 peer-reviewed journals. Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere. Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates. Read from thousands of the leading scholarly journals from Springer, Elsevier, Nature, IEEE, Wiley-Blackwell and more. To subscribe to email alerts, please log in first, or sign up for a DeepDyve account if you don’t already have one. To get new article updates from a journal on your personalized homepage, please log in first, or sign up for a DeepDyve account if you don’t already have one. The ability of the Hermes transposable element to function as a germ line transformation vector was tested in the stable fly, Stomoxys calcitrans. Plasmid‐based transposable element mobility assays indicated moderate mobility of Hermes in this species. Germline transformants were created using a Hermes element containing the enhanced green fluorescent protein (EGFP) under the regulatory control of the promoter from Actin5C gene of Drosophila melanogaster. Approximately 4% of the fifty‐five adults that developed from the 1903 G0 embryos injected with the vector produced transgenic progeny. In the four transgenic lines established, the EGFP expression pattern was distinctly nonuniform and levels of expression were low. Promoters other than the one from the Actin5C gene of D.melanogaster should be considered for widespread, constitutive expression. All transgenic lines contained multiple (2–4) integrated Hermes elements. Hermes integration events occurred through a canonical cut‐and‐paste mechanism. “Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.” Source.


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